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Regarding small vertebrates, only two case studies were found: the Late Prehistoric dispersal of Polynesians to New Zealand was dated using the commensal Pacific rat as a proxy. ivory, bone or antler), progress in sample pretreatments using ultrafiltration.

In practice, the manipulation of small bone samples presents several obstacles which are difficult to overcome, especially for ancient (Middle to Upper Paleolithic transition) samples.An optimized protocol allowed us to extract enough material to produce between 0.2 and 1.0 mg of carbon for graphite targets.Our approach was tested on known-age samples dating back to 40,000 BP, and served as proof of concept.The main consensus in the radiocarbon community is that bones with less than a 1% collagen yield should not be dated C measurement is the 1% collagen yield threshold.Considering that collagen contains about 40–45% carbon, 250 mg of bone are necessary to provide enough carbon for a regular-sized graphite target of 1 mg.

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